Immunofluorescent analysis of BrdU (green) showing staining in the nucleus of Hela cells treated with 100nM BrdU (right) and untreated Hela cells (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a BrdU monoclonal antibody (Product # MA3-071) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Supplier Page from Thermo Fisher Scientific for BrdU Antibody